150 nm nanoparticles using the emulsification-diffusion technique in order to perform sterilization by filtration using 0. The influence of the copolymer molar parenteral technology manual interpharm press usa pdf and the theoretical drug loading was investigated in terms of particle size, drug loading, entrapment efficiency and surface characteristics.
With all the polymers used, sub-150 nm nanoparticles were produced with good reproducibility and narrow size distributions irrespective of both the polymer nature and the theoretical drug loading. For all types of nanoparticles, complete redispersion in various media could be obtained. All final freeze-dried products were refiltrable on a 0. 22 μm membrane and were stable in terms of mean particle size and drug loading over a period up to 6 months.
The effective drug loading increased at higher theoretical drug loading, the entrapment efficiency was however decreased. The same trend was observed with the three polyesters. The sterility of the final freeze-dried nanoparticles was confirmed by the results of the sterility testing which showed no bacterial contamination. Check if you have access through your login credentials or your institution. Present address: Bracco Research S. Screen reader users, click the load entire article button to bypass dynamically loaded article content. Please note that Internet Explorer version 8.
Click the View full text link to bypass dynamically loaded article content. Important aspects of working with microorganisms in the laboratory and maintaining them for future use include assuring their integrity, handling them safely, and complying with regulations for safe and secure storage and transfer. Integrity can be assured by using traditional methods of preservation, low-temperature storage, and packaging systems for protecting the material during transport. Cultures can be deliberately manipulated to create a state of dormancy by freezing or freeze-drying, and by maintaining conditions during handling and transport that assure continued viability and efficacy of the microorganism.
Handling and maintenance of hazardous microorganisms requires attention to good biosafety and biosecurity practices. Infectious agents present risks to scientists and technicians who handle them, and depending on the agent they can present more widespread public health risks, as well as environmental and economical risks by infecting plants and animals. Good safety practices and controls are necessary to ensure that these risks are minimized and biosecurity measures assure that dual-use microorganisms are not accessible to those intending to use them for nefarious purposes. Compliance with US and international regulations for handling, storage, and transport of infectious agents is a critical part of working with hazardous microbial cultures in the current environment of heightened awareness of the potential for misuse of microbiological agents. This article has not been cited. 150 nm nanoparticles using the emulsification-diffusion technique in order to perform sterilization by filtration using 0. The influence of the copolymer molar ratio and the theoretical drug loading was investigated in terms of particle size, drug loading, entrapment efficiency and surface characteristics.